Anti-160 kD Neurofilament Medium抗体[NF-09] (ab7794)

概述

  • 产品名称Anti-160 kD Neurofilament Medium抗体[NF-09]
    参阅全部 160 kD Neurofilament Medium 一抗
  • 描述
    小鼠单克隆抗体[NF-09] to 160 kD Neurofilament Medium
  • 特异性This antibody reacts with both phosphorylated and non-phosphorylated forms of medium neurofilament protein (160 kDa) of various species.
  • 经测试应用适用于: ELISA, IHC-Fr, ICC, IHC-P, WB, IHC (PFA fixed), ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Cat, Human, Pig
    预测可用于: a wide range of other species, all Mammals
  • 免疫原

    Pellet of pig brain cold stable proteins after depolymerization of microtubules.

性能

应用

Our Abpromise guarantee covers the use of ab7794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB 1/1000.
IHC (PFA fixed) 1/500.
ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
  • 细胞定位Cytoplasm
  • 数据库链接
  • 别名
    • 150kDa medium antibody
    • NEF3 antibody
    • NEFM antibody
    • Neurofilament 3 antibody
    • NF160 antibody
    • NFM antibody
    see all

Anti-160 kD Neurofilament Medium antibody [NF-09] 图像

  • ab7794 at a dilution of 1/1000, staining 160 kD Neurofilament Medium (Alexa 488 secondary at 1/2000) on rat brain tissue (30µm thick coronal sections) in free floating IHC (see protocol link for detailed description). Image taken with a 40x objective. No labeling observed following omission of primary antibody.

    Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

  • ICC/IF image of ab7794 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7794, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

  • ab7794 staining Neurofilament medium protein in mouse Neuro2A cells by ICC/IF.
  • Immunofluorescence analysis of 1 month neuronal-differentiated dental pulp stem cells, staining 160 kD Neurofilament Medium with ab7794.

    Cells were fixed with paraformaldehyde and incubated with primary antibody (1/600). A FITC-conjugated anti-mouse IgG (1/375) was used as the secondary antibody.

实验方案

Anti-160 kD Neurofilament Medium antibody [NF-09] (ab7794)参考文献

This product has been referenced in:
  • Guven S  et al. Functional maintenance of differentiated embryoid bodies in microfluidic systems: a platform for personalized medicine. Stem Cells Transl Med 4:261-8 (2015). ICC/IF ; Mouse . Read more (PubMed: 25666845) »
  • Matsumura S  et al. Characterization of mesenchymal progenitor cell populations from non-epithelial oral mucosa. Oral Dis N/A:N/A (2014). Read more (PubMed: 25180458) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (nerve)
Specification nerve
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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提交于 Jun 23 2010

The originator of ab7794 uses cell brain lysate (pig or mouse) as a positive control in Western blots. If you have any more questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"