Anti-160 kD Neurofilament Medium抗体(ab30309)

概述

  • 产品名称Anti-160 kD Neurofilament Medium抗体
    参阅全部 160 kD Neurofilament Medium 一抗
  • 描述
    兔多克隆抗体to 160 kD Neurofilament Medium
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Chicken, Human
    预测可用于: all Mammals
  • 免疫原

    Recombinant C-terminal fragment (Rat)

性能

应用

Our Abpromise guarantee covers the use of ab30309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 20635090
ICC/IF 1/100 - 1/1000.
WB 1/1000 - 1/5000. Predicted molecular weight: 102 kDa.

靶标

  • 相关性Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
  • 细胞定位Cytoplasm
  • 数据库链接
  • 别名
    • 150kDa medium antibody
    • NEF3 antibody
    • NEFM antibody
    • Neurofilament 3 antibody
    • NF160 antibody
    • NFM antibody
    see all

Anti-160 kD Neurofilament Medium antibody (ab30309)参考文献

This product has been referenced in:
  • Seidel K  et al. Axonal inclusions in spinocerebellar ataxia type 3. Acta Neuropathol 120:449-60 (2010). IHC-P ; Human . Read more (PubMed: 20635090) »
  • Zhao X  et al. Neuronal PPARgamma deficiency increases susceptibility to brain damage after cerebral ischemia. J Neurosci 29:6186-95 (2009). WB ; Mouse . Read more (PubMed: 19439596) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human Brain)
Loading amount 15 µg
Specification Human Brain
Gel Running Conditions Reduced Denaturing (12%T)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Daniel Martins-de-Souza

Verified customer

提交于 Feb 25 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"