Western blot - Anti-15 Lipoxygenase 2 antibody (ab23691)
All lanes : Anti-15 Lipoxygenase 2 antibody (ab23691) at 4 µg/ml
Lane 1 : Rabbit reticulocyte lysate (30 µg). Lane 2 : Human prostate homogenate (30 µg).
Predicted band size: 76 kDa Observed band size: 76 kDa
Immunohistochemistry (Frozen sections) - Anti-15 Lipoxygenase 2 antibody (ab23691)This image is courtesy of an anonymous Abreview
ab23691 staining 15 Lipoxygenase 2 in human artery tissue sections by IHC-Fr (frozen sections). Tissue samples were fixed with acetone and blocked with 0.5% BSA for 10 minutes. Samples were incubated with primary antibody 1/100 24 hours. A biotin-conjugated horse anti Rabbit IgG (H+L) polyclonal (7 µg/ml) was used as secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-15 Lipoxygenase 2 antibody (ab23691)This image is courtesy of an anonymous Abreview
Immunofluorescence analysis of murine embryonic fibroblasts, staining 15 Lipoxygenase 2 with ab23691.
Cells were fixed with paraformaldehyde, permeabilized with 0.2 % Triton-X-100 and blocked with 2% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/100 in 1% BSA in TBST) for 16 hours at 4°C. An AlexaFluor®488-conjugated chicken anti-rabbit polyclonal IgG (1/2000) was used as the secondary antibody.
Bender G et al. Membrane-dependent Activities of Human 15-LOX-2 and Its Murine Counterpart: IMPLICATIONS FOR MURINE MODELS OF ATHEROSCLEROSIS. J Biol Chem291:19413-24 (2016).
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