概述

  • 产品名称Anti-15 Lipoxygenase 1抗体
    参阅全部 15 Lipoxygenase 1 一抗
  • 描述
    兔多克隆抗体to 15 Lipoxygenase 1
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat
  • 免疫原

    Synthetic peptide corresponding to Mouse 15 Lipoxygenase 1 aa 50-150 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P39654
    (Peptide available as ab139428)

  • 阳性对照
    • WB: Raw264.7 and EL4 whole cell lysates and mouse kidney, heart, small intestine and brain tissue lysates. ICC/IF: RAW246.7 cells.

性能

应用

Our Abpromise guarantee covers the use of ab87353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).

靶标

  • 功能Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid.
  • 通路Lipid metabolism; leukotriene D4 biosynthesis.
  • 序列相似性Belongs to the lipoxygenase family.
    Contains 1 lipoxygenase domain.
    Contains 1 PLAT domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 15 lipoxygenase 1 antibody
    • 15 LIPOXYGENASE RETICULOCYTE ARACHIDONATE antibody
    • 15 LOX antibody
    • 15 LOX 1 antibody
    • 15-LOX antibody
    • 15LOX 1 antibody
    • ALOX15 antibody
    • Arachidonate 15 lipoxygenase antibody
    • Arachidonate 15-lipoxygenase antibody
    • Arachidonate omega 6 lipoxygenase antibody
    • Arachidonate omega-6 lipoxygenase antibody
    • LOG15 antibody
    • LOX15_HUMAN antibody
    see all

Anti-15 Lipoxygenase 1 antibody 图像

  • ICC/IF image of ab87353 stained RAW246.7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87353, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-15 Lipoxygenase 1 antibody (ab87353) at 1 µg/ml

    Lane 1 : Kidney (Mouse) Tissue Lysate
    Lane 2 : Heart (Mouse) Tissue Lysate
    Lane 3 : Small Intestine (Mouse) Tissue Lysate
    Lane 4 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
    Lane 5 : Brain (Mouse) Tissue Lysate
    Lane 6 : Forebrain (Mouse) Tissue Lysate
    Lane 7 : EL4 whole cell lysate (ab7183)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 75 kDa
    Observed band size : 75 kDa
    Additional bands at : 41 kDa,74 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

Anti-15 Lipoxygenase 1 antibody (ab87353)参考文献

This product has been referenced in:
  • Suzuki H  et al. Arachidonate 12/15-lipoxygenase-induced inflammation and oxidative stress are involved in the development of diabetic cardiomyopathy. Diabetes 64:618-30 (2015). Mouse . Read more (PubMed: 25187369) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (10)
Sample Mouse Tissue lysate - whole (Lung)
Specification Lung
Treatment 2ppm ozone for 3weeks
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Dec 05 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"