概述

  • 产品名称
    Anti-14-3-3 zeta抗体
    参阅全部 14-3-3 zeta 一抗
  • 描述
    兔多克隆抗体to 14-3-3 zeta
  • 特异性
    ab51129 detects endogenous levels of total 14-3-3 zeta protein.
  • 经测试应用
    适用于: ELISA, WB, ICC, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthesized non-phosphopeptide derived from human 14-3-3 zeta around the phosphorylation site of serine 58 (R-S-SP-W-R).

  • 阳性对照
    • Human breast carcinoma tissue or extracts from 293 cells treated with Forskolin.

性能

应用

Our Abpromise guarantee covers the use of ab51129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
ICC
ICC/IF
IHC-P
  • 应用说明
    ELISA: 1/40000.
    ICC: Use at a concentration of 1 µg/ml.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately ~28 kDa (predicted molecular weight: 28 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
    • 序列相似性
      Belongs to the 14-3-3 family.
    • 翻译后修饰
      The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.
    • 细胞定位
      Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 14 3 3 delta antibody
      • 14 3 3 protein zeta/delta antibody
      • 14 3 3 protein/cytosolic phospholipase A2 antibody
      • 14 3 3 zeta antibody
      • 14-3-3 protein zeta/delta antibody
      • 1433Z_HUMAN antibody
      • Epididymis luminal protein 4 antibody
      • Epididymis secretory protein Li 3 antibody
      • HEL S 3 antibody
      • HEL4 antibody
      • KCIP-1 antibody
      • KCIP1 antibody
      • MGC111427 antibody
      • MGC126532 antibody
      • MGC138156 antibody
      • Phospholipase A2 antibody
      • Protein kinase C inhibitor protein 1 antibody
      • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide antibody
      • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta antibody
      • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody
      • Tyrosine 3/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody
      • YWHAD antibody
      • YWHAZ antibody
      see all

    图片

    • All lanes : Anti-14-3-3 zeta antibody (ab51129)

      Lane 1 : Extracts from 293 cells treated with Forskolin (40nM, 30min) with no peptide
      Lane 2 : Extracts from 293 cells treated with Forskolin (40nM, 30min) with immunising peptide


      Predicted band size : 28 kDa
      Observed band size : 28 kDa
    • ab51129 staining 14-3-3 zeta in Human glioblastoma samples (A) and low grade tumors (B) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) overnight. Nuclei stained blue with hematoxylin. Scale bars = 20 µm


    • Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 28 kDa


      Exposure time : 4 minutes
    • This image shows paraffin-embedded human breast carcinoma tissue stained with ab51129 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue
    • ICC/IF image of ab51129 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51129, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    文献

    This product has been referenced in:
    • Piersma B  et al. YAP1 Is a Driver of Myofibroblast Differentiation in Normal and Diseased Fibroblasts. Am J Pathol 185:3326-37 (2015). Read more (PubMed: 26458763) »
    • Simeone P  et al. A unique four-hub protein cluster associates to glioblastoma progression. PLoS One 9:e103030 (2014). IHC-P ; Human . Read more (PubMed: 25050814) »

    See all 7 Publications for this product

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Breast Cancer cell line (MCF7))
    Loading amount
    20 µg
    Specification
    Breast Cancer cell line (MCF7)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 06 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (macrophage)
    Loading amount
    20 µg
    Specification
    macrophage
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 17 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Colorectal cancer)
    Loading amount
    30 µg
    Specification
    Colorectal cancer
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Dr. Lay Harn Gam

    Verified customer

    提交于 May 19 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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