RabMAb

Anti-14-3-3 Theta + Tau抗体[EPR5123] (ab124909)

概述

  • 产品名称Anti-14-3-3 Theta + Tau抗体[EPR5123]
  • 描述
    兔单克隆抗体[EPR5123] to 14-3-3 Theta + Tau
  • 经测试应用适用于: ICC/IF, WB, Flow Cytmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human 14-3-3 Theta + Tau (N terminal). The exact sequence is proprietary.

  • 阳性对照
    • HeLa, 293T, A549 and A431 cell lysates; A549 cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab124909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/50 - 1/100.
WB 1/10000 - 1/50000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
Flow Cyt 1/500 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for IHC-P or IP.
  • 靶标

    • 功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
    • 组织特异性Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
    • 序列相似性Belongs to the 14-3-3 family.
    • 翻译后修饰Ser-232 is probably phosphorylated by CK1.
    • 细胞定位Cytoplasm. In neurons, axonally transported to the nerve terminals.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 14-3-3 protein T cell antibody
      • 14-3-3 protein T-cell antibody
      • 14-3-3 protein tau antibody
      • 14-3-3 protein theta antibody
      • 1433T_HUMAN antibody
      • IC5 antibody
      • Protein HS1 antibody
      • Protein tau antibody
      • YWHAQ tyrosine 3 monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide antibody
      • Ywhaq antibody
      see all

    Anti-14-3-3 Theta + Tau antibody [EPR5123] 图像

    • All lanes : Anti-14-3-3 Theta + Tau antibody [EPR5123] (ab124909) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 28 kDa
      Observed band size : 28 kDa
    • Flow cytometric analysis of permeablized A549 cells using ab124909 at 1/500 dilution (red) or a rabbit IgG negative control (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-14-3-3 Theta + Tau antibody [EPR5123] (ab124909)参考文献

    ab124909 has not yet been referenced specifically in any publications.

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