概述

  • 产品名称
    Anti-14-3-3 Tau抗体
    参阅全部 14-3-3 Tau 一抗
  • 描述
    小鼠多克隆抗体to 14-3-3 Tau
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Rabbit, Chicken, Cow, Human, Xenopus laevis, Cynomolgus monkey, Orangutan
  • 免疫原

    Fusion protein corresponding to Mouse 14-3-3 Tau aa 51-151. Vector coding for a partial recombinant fusion protein, corresponding to amino acids 51-151 of Mouse 14-3-3 Tau.
    Sequence:

    VVGGRRSAWRVISSIEQKTDTSDKKLQLIKDYREKVESELRSICTTVLEL LDKYLIANATNPESKVFYLKMKGDYFRYLAEVACGDDRKQTIENSQGAYQ


    Database link: P68254

  • 常规说明

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 纯度
    Whole antiserum
  • Primary antibody说明
    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 28 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 功能
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
  • 组织特异性
    Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
  • 序列相似性
    Belongs to the 14-3-3 family.
  • 翻译后修饰
    Ser-232 is probably phosphorylated by CK1.
  • 细胞定位
    Cytoplasm. In neurons, axonally transported to the nerve terminals.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 14 3 3 protein T cell antibody
    • 14 3 3 protein tau antibody
    • 14 3 3 protein theta antibody
    • 14 3 3 tau antibody
    • 14 3 3 theta antibody
    • 14-3-3 protein T-cell antibody
    • 14-3-3 protein tau antibody
    • 14-3-3 protein theta antibody
    • 1433T_HUMAN antibody
    • 1C5 antibody
    • HS1 antibody
    • KCIP1 antibody
    • Protein HS1 antibody
    • Protein kinase C inhibitor protein 1 antibody
    • Protein tau antibody
    • tyr3/trp5 monooxygenase activation protein, theta antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, theta polypeptide antibody
    • Ywhaq antibody
    see all

Anti-14-3-3 Tau antibody 图像

  • All lanes : Anti-14-3-3 Tau antibody (ab52834) at 1/1000 dilution

    Lane 1 : a total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigen
    Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (tagged fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated, at 1/5000 dilution
    Lane 2 : Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated, at 1/5000 dilution


    Predicted band size : 28 kDa

实验方案

Anti-14-3-3 Tau antibody (ab52834)参考文献

This product has been referenced in:
  • Fang M  et al. Bioinformatics and co-expression network analysis of differentially expressed lncRNAs and mRNAs in hippocampus of APP/PS1 transgenic mice with Alzheimer disease. Am J Transl Res 9:1381-1391 (2017). IHC-P ; Mouse . Read more (PubMed: 28386363) »

See 1 Publication for this product

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