Anti-14-3-3 sigma抗体(ab89405)

概述

  • 产品名称Anti-14-3-3 sigma抗体
    参阅全部 14-3-3 sigma 一抗
  • 描述
    小鼠单克隆抗体to 14-3-3 sigma
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length Human 14-3-3 sigma protein (AAH00329) with a proprietary tag.

  • 阳性对照
    • Human ovary cancer tissue lysate. Tagged recombinant Human 14-3-3 sigma protein.

性能

应用

Our Abpromise guarantee covers the use of ab89405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 28 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged 14-3-3 sigma is approximately 0.3ng/ml when used as a capture antibody.

靶标

  • 功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
    p53-regulated inhibitor of G2/M progression.
  • 组织特异性Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
  • 序列相似性Belongs to the 14-3-3 family.
  • 翻译后修饰Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
  • 细胞定位Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 14 3 3 protein sigma antibody
    • 14-3-3 protein sigma antibody
    • 1433S_HUMAN antibody
    • Epithelial cell marker protein 1 antibody
    • Er antibody
    • HME 1 antibody
    • HME1 antibody
    • MGC143283 antibody
    • Mkrn3 antibody
    • Mme1 antibody
    • OTTHUMP00000004242 antibody
    • RP23 137L22.11 antibody
    • SFN antibody
    • SFN protein antibody
    • Stratifin antibody
    • YWHAS antibody
    see all

Anti-14-3-3 sigma antibody 图像

  • Anti-14-3-3 sigma antibody (ab89405) at 5 µg/ml + Human ovary cancer tissue lysate at 50 µg

    Predicted band size : 28 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • Anti-14-3-3 sigma antibody (ab89405) at 5 µg/ml + Tagged recombinant Human 14-3-3 sigma protein at 0.2 µg

    Predicted band size : 28 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
  • Detection limit for recombinant tagged 14-3-3 sigma is approximately 0.3ng/ml as a capture antibody.

Anti-14-3-3 sigma antibody (ab89405)参考文献

ab89405 has not yet been referenced specifically in any publications.

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