Anti-14-3-3 sigma抗体(ab86380)


  • 产品名称Anti-14-3-3 sigma抗体
    参阅全部 14-3-3 sigma 一抗
  • 描述
    兔多克隆抗体to 14-3-3 sigma
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between amino acids 198 and 248 of Human 14-3-3 sigma (NP_006133.1)

  • 阳性对照
    • Hela cell lysate




Our Abpromise guarantee covers the use of ab86380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 28 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • 功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
    p53-regulated inhibitor of G2/M progression.
  • 组织特异性Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
  • 序列相似性Belongs to the 14-3-3 family.
  • 翻译后修饰Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
  • 细胞定位Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 14 3 3 protein sigma antibody
    • 14-3-3 protein sigma antibody
    • 1433S_HUMAN antibody
    • Epithelial cell marker protein 1 antibody
    • Er antibody
    • HME 1 antibody
    • HME1 antibody
    • MGC143283 antibody
    • Mkrn3 antibody
    • Mme1 antibody
    • OTTHUMP00000004242 antibody
    • RP23 137L22.11 antibody
    • SFN antibody
    • SFN protein antibody
    • Stratifin antibody
    • YWHAS antibody
    see all

Anti-14-3-3 sigma antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling 14-3-3 sigma with ab86380 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-14-3-3 sigma antibody (ab86380) at 0.1 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg
    Lane 3 : Hela whole cell lysate at 5 µg

    developed using the ECL technique

    Predicted band size : 28 kDa

    Exposure time : 30 seconds
  • Detection of 14-3-3 sigma by Western blot of Immunprecipitate.
    ab86380, at 1 µg/ml, staining 14-3-3 sigma in HeLa whole cell lysate immunoprecipitated using ab86380 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-14-3-3 sigma antibody (ab86380)参考文献

ab86380 has not yet been referenced specifically in any publications.

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