1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53. p53-regulated inhibitor of G2/M progression.
组织特异性Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
序列相似性Belongs to the 14-3-3 family.
翻译后修饰Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
细胞定位Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
Detection of 14-3-3 sigma by Western blot of Immunprecipitate.
ab86380, at 1 µg/ml, staining 14-3-3 sigma in HeLa whole cell lysate immunoprecipitated using ab86380 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.
Anti-14-3-3 sigma antibody (ab86380)参考文献
has not yet been referenced specifically in any publications.