The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
Use a concentration of 2 - 4 µg/ml.
Use at an assay dependent concentration.
Antibody detection limit dilution 1:16,000.
Use at an assay dependent concentration. PubMed: 25298405
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53. p53-regulated inhibitor of G2/M progression.
Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
Belongs to the 14-3-3 family.
Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
Immunohistochemical analysis of paraffin embedded Human Skin tissue labeling 14-3-3 sigma with ab77187 at 2 ug/ml. Tissue underwent antigen retrieval in steam with Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.
Western blot - Anti-14-3-3 sigma antibody (ab77187)
Anti-14-3-3 sigma antibody (ab77187) at 0.1 µg/ml + human skin lysate in RIPA buffer at 35 µg
Predicted band size: 28 kDa Observed band size: 28 kDa
Immunohistochemical analysis of paraffin embedded Human Skin tissue labeling 14-3-3 sigma with ab77187 at 2.5 ug/ml. Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.