概述

  • 产品名称Anti-14-3-3 sigma抗体
    参阅全部 14-3-3 sigma 一抗
  • 描述
    山羊多克隆抗体to 14-3-3 sigma
  • 经测试应用适用于: WB, IHC-P, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Cow, Dog, Pig, Zebrafish, Dictyostelium discoideum, Rainbow Trout
  • 免疫原

    Synthetic peptide corresponding to Human 14-3-3 sigma (internal sequence).
    Sequence:

    DLHTLSEDSYKDST


    Database link: P31947

  • 阳性对照
    • Human skin lysates. Human skin tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab77187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IHC-P Use a concentration of 2 - 4 µg/ml.
ELISA Use at an assay dependent concentration.

Antibody detection limit dilution 1:16,000.

ICC/IF Use at an assay dependent concentration. PubMed: 25298405

靶标

  • 功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
    p53-regulated inhibitor of G2/M progression.
  • 组织特异性Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
  • 序列相似性Belongs to the 14-3-3 family.
  • 翻译后修饰Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
  • 细胞定位Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 14 3 3 protein sigma antibody
    • 14-3-3 protein sigma antibody
    • 1433S_HUMAN antibody
    • Epithelial cell marker protein 1 antibody
    • Er antibody
    • HME 1 antibody
    • HME1 antibody
    • MGC143283 antibody
    • Mkrn3 antibody
    • Mme1 antibody
    • OTTHUMP00000004242 antibody
    • RP23 137L22.11 antibody
    • SFN antibody
    • SFN protein antibody
    • Stratifin antibody
    • YWHAS antibody
    see all

Anti-14-3-3 sigma antibody 图像

  • Immunohistochemical analysis of paraffin embedded Human Skin tissue labeling 14-3-3 sigma with ab77187 at 2 ug/ml. Tissue underwent antigen retrieval in steam with Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.

  • Anti-14-3-3 sigma antibody (ab77187) at 0.1 µg/ml + human skin lysate in RIPA buffer at 35 µg

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
  • Immunohistochemical analysis of paraffin embedded Human Skin tissue labeling 14-3-3 sigma with ab77187 at 2.5 ug/ml. Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

Anti-14-3-3 sigma antibody (ab77187)参考文献

This product has been referenced in:
  • Plak K  et al. Rap1-dependent pathways coordinate cytokinesis in Dictyostelium. Mol Biol Cell 25:4195-204 (2014). ICC/IF ; Dictyostelium discoideum . Read more (PubMed: 25298405) »

See 1 Publication for this product

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