Anti-14-3-3 sigma/SFN抗体(ab151504)
Key features and details
- Rabbit polyclonal to 14-3-3 sigma/SFN
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-14-3-3 sigma/SFN抗体
参阅全部 14-3-3 sigma/SFN 一抗 -
描述
兔多克隆抗体to 14-3-3 sigma/SFN -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human 14-3-3 sigma/SFN aa 154-247.
Database link: P31947 -
阳性对照
- 293T, A431, HeLa, HepG2, Neuro2A, GL261 and PC12 whole cell lysates; A431 cells; Human 59T xenograft tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab151504于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 27 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated retrieval with Tris-EDTA buffer pH 8
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 27 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated retrieval with Tris-EDTA buffer pH 8 |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
p53-regulated inhibitor of G2/M progression. -
组织特异性
Present mainly in tissues enriched in stratified squamous keratinizing epithelium. -
序列相似性
Belongs to the 14-3-3 family. -
翻译后修饰
Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation. -
细胞定位
Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway. - Information by UniProt
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数据库链接
- Entrez Gene: 2810 Human
- Entrez Gene: 55948 Mouse
- Entrez Gene: 313017 Rat
- Omim: 601290 Human
- SwissProt: P31947 Human
- SwissProt: O70456 Mouse
- Unigene: 523718 Human
- Unigene: 44482 Mouse
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别名
- 14 3 3 protein sigma antibody
- 14-3-3 protein sigma antibody
- 1433S_HUMAN antibody
see all
图片
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All lanes : Anti-14-3-3 sigma/SFN antibody (ab151504) at 1/3000 dilution
Lane 1 : Neuro2A whole cell lysate
Lane 2 : GL261 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 27 kDa
12% SDS PAGE -
Anti-14-3-3 sigma/SFN antibody (ab151504) at 1/3000 dilution + PC12 whole cell lysate at 30 µg
Predicted band size: 27 kDa
12% SDS PAGE -
All lanes : Anti-14-3-3 sigma/SFN antibody (ab151504) at 1/3000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 27 kDa
12% SDS PAGE -
Immunofluorescent analysis of A431 cells (fixed in ice-cold Methanol for 5 min), labeling 14-3-3 sigma/SFN with ab151504 at 1/500 dilution. The right image shows cells co-stained with Hoechst 33343.
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Immunohistochemical analysis of paraffin-embedded Human 59T xenograft tissue, labeling 14-3-3 sigma/SFN with ab151504 at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab151504 尚未被引用在任何文献中。