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Recombinant full length protein (Human).
Our Abpromise guarantee covers the use of ab14123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. Use 2µg/mg protein lysate. Use protein G.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. 2 hours at RT.|
|IHC-P||Use a concentration of 2 - 4 µg/ml.|
|ICC/IF||Use a concentration of 1 µg/ml.|
ab14123 staining mouse embryonic skin tissue sections by IHC-P. Sections were PFA fixed and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking with a commercially available (mouse-on-mouse) blocking reagent for 1 hour at RT. The primary antibody was diluted 1/50 and incubated with the sample for 24 hours at 4°C. A biotinylated mouse anti-mouse antibody was used as the secondary, followed by a Streptavidin-Cy3®.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"