Anti-14-3-3 sigma抗体[1.N.6] (ab14123)

概述

  • 产品名称Anti-14-3-3 sigma抗体[1.N.6]
    参阅全部 14-3-3 sigma 一抗
  • 描述
    小鼠单克隆抗体[1.N.6] to 14-3-3 sigma
  • 特异性Recognizes human 14-3-3 sigma protein, 26-30kD. Does not crossreact with other isoforms of 14-3-3.
  • 经测试应用适用于: IP, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein (Human).

  • 阳性对照
    • HT29 cells and skin

性能

应用

Our Abpromise guarantee covers the use of ab14123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration. Use 2µg/mg protein lysate. Use protein G.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. 2 hours at RT.
IHC-P Use a concentration of 2 - 4 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
    p53-regulated inhibitor of G2/M progression.
  • 组织特异性Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
  • 序列相似性Belongs to the 14-3-3 family.
  • 翻译后修饰Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
  • 细胞定位Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 14 3 3 protein sigma antibody
    • 14-3-3 protein sigma antibody
    • 1433S_HUMAN antibody
    • Epithelial cell marker protein 1 antibody
    • Er antibody
    • HME 1 antibody
    • HME1 antibody
    • MGC143283 antibody
    • Mkrn3 antibody
    • Mme1 antibody
    • OTTHUMP00000004242 antibody
    • RP23 137L22.11 antibody
    • SFN antibody
    • SFN protein antibody
    • Stratifin antibody
    • YWHAS antibody
    see all

Anti-14-3-3 sigma antibody [1.N.6] 图像

  • ab14123 staining mouse embryonic skin tissue sections by IHC-P.  Sections were PFA fixed and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking with a commercially available (mouse-on-mouse) blocking reagent for 1 hour at RT.  The primary antibody was diluted 1/50 and incubated with the sample for 24 hours at 4°C.   A biotinylated mouse anti-mouse antibody was used as the secondary, followed by a Streptavidin-Cy3®. 

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  • All lanes : Anti-14-3-3 sigma antibody [1.N.6] (ab14123) at 0.5 µg/ml

    Lane 1 : U251 (non malignant glioma cells)
    Lane 2 : CFBE41o- cells stably transfected with wild-type cystic fibrosis transmembrane conductance regulator (CFTR).
    Lane 3 : CFBE41o- cells stably transfected with deltaF508 of cystic fibrosis transmembrane conductance regulator (CFTR).

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti mouse (HRP) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 30 kDa

    See Abreview

  • ab14123 at 1/50 staining human epithelium tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step was performed. The tissue was incubated with the antibody for 30 minutes. An HRP conjugated goat polyclonal antibody was used as the secondary.

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  • ICC/IF image of ab14123 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14123, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-14-3-3 sigma antibody [1.N.6] (ab14123)参考文献

This product has been referenced in:
  • Zakharchenko O  et al. A role of TGFß1 dependent 14-3-3s phosphorylation at Ser69 and Ser74 in the regulation of gene transcription, stemness and radioresistance. PLoS One 8:e65163 (2013). Human . Read more (PubMed: 23741479) »
  • Skibinski CG  et al. Proteomic changes induced by effective chemopreventive ratios of n-3:n-6 fatty acids and tamoxifen against MNU-induced mammary cancer in the rat. Cancer Prev Res (Phila) 6:979-88 (2013). Read more (PubMed: 23880232) »

See all 13 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tongue)
Specification tongue
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM Tris pH=9.0 / 1 mM EDTA
Permeabilization No
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Abcam user community

Verified customer

提交于 Feb 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (head and neck cancer)
Specification head and neck cancer
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH=6.0
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
Username

Dr. satyendra tripathi

Verified customer

提交于 Jul 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A549 cells)
Loading amount 10000 cells
Specification A549 cells
Treatment 26 nM DMSO/bortezomib (BTZ) 24 hours
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (embryonic skin)
Specification embryonic skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Blocking step Vector M.O.M Kit as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Feb 29 2008

Thank you for your enquiry. This product, ab14123 was generated with Recombinant human 14-3-3 sigma protein (26-30kD) as the immunogen. Unfortunately, there is no further epitope information available. I am sorry I can't be of more assistance i...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (epithelium)
Specification epithelium
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Username

Mr. Rudolf Jung

Verified customer

提交于 Feb 28 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (Hela, Jurkat and SupT1 cell lines)
Loading amount 10 µg
Specification Hela, Jurkat and SupT1 cell lines
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Nov 13 2006

Thank you for your enquiry. Both BSA and glycerol stabilize the antibody. BSA is a carrier protein, and is added to increase the protein concentration of the product, as this helps stabilize the material. Glycerol is a cryoprotectant that prevents...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U251 glial cells; CFBE41o- airway epithelium)
Specification U251 glial cells; CFBE41o- airway epithelium
Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Oct 10 2005

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HCT 116)
Specification HCT 116
Immuno-precipitation step Protein A/G
Username

Abcam user community

Verified customer

提交于 Sep 12 2005

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