Anti-14-3-3 (phospho S58)抗体(ab30554)

概述

  • 产品名称Anti-14-3-3 (phospho S58)抗体
    参阅全部 14-3-3 一抗
  • 描述
    兔多克隆抗体to 14-3-3 (phospho S58)
  • 特异性Specific for the ~29k 14-3-3 protein phosphorylated at Ser58. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
  • 经测试应用适用于: WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Zebrafish
    预测可用于: Sheep, Chicken, Cow, Dog, Xenopus laevis, Non Human Primates
  • 免疫原

    Phosphopeptide corresponding to amino acid residues surrounding the phosphorylation site Ser58 of rat 14-3-3 protein.

性能

应用

Our Abpromise guarantee covers the use of ab30554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 29 kDa.
IHC-Fr 1/500.

靶标

Anti-14-3-3 (phospho S58) antibody 图像

  • All lanes : Anti-14-3-3 (phospho S58) antibody (ab30554) at 1/1000 dilution

    Lane 1 : Rat brain stem lysate
    Lane 2 : Rat brain stem lysate with the phosphopeptide used as the antigen


    Observed band size : 29 kDa (why is the actual band size different from the predicted?)
  • ab30554 staining 14-3-3 (phospho S58) in Mouse hippocampal tissue by Immunohistochemistry (Frozen sections). ab30554 is green, red staining is GFAP and nuclei are stained blue with DAPI.
  • ab30554 staining 14-3-3 (phospho S58) in human U2OS cells by Immunocytochemistry/ Immunofluorescence.
    U2OS cells were plated on coverslips and fixed by sequential incubations with 4% formaldehyde in PBS without calcium and magnesium [PBS(-)], 0.1% Triton X-100 in PBS(-) and 0.05% Tween-20 in PBS(-), each for 10 minutes at room temperature. After being washed, cells were incubated with ab30554, followed by incubation with Alexa Fluor 488–conjugated anti-rabbit IgG in TBST (100 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.05% Tween-20) containing 5% FBS.
  • All lanes : Anti-14-3-3 (phospho S58) antibody (ab30554) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate (20ug)
    Lane 3 : Mouse brain homogenate (20ug)

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 28 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

Anti-14-3-3 (phospho S58) antibody (ab30554)参考文献

This product has been referenced in:
  • Okada N  et al. A novel Chk1/2-Lats2-14-3-3 signaling pathway regulates P-body formation in response to UV damage. J Cell Sci 124:57-67 (2011). WB, ICC/IF ; Human . Read more (PubMed: 21118956) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Sample Mouse Cell (Mouse colon.)
Specification Mouse colon.
Permeabilization No
Fixative PFA/Ethanol
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Abcam user community

Verified customer

提交于 Aug 01 2014

Thank you for your enquiry. I have been in touch with the source of this antibody and I have determined that by immunoblotting this antibody is blocked by the phosphopeptide used as the antigen but NOT by the corresponding dephosphopeptide. We ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"