Anti-14-3-3 (phospho S58)抗体(ab30554)
Key features and details
- Rabbit polyclonal to 14-3-3 (phospho S58)
- Suitable for: WB, IHC-Fr
- Reacts with: Mouse, Rat, Zebrafish
- Isotype: IgG
概述
-
产品名称
Anti-14-3-3 (phospho S58)抗体
参阅全部 14-3-3 一抗 -
描述
兔多克隆抗体to 14-3-3 (phospho S58) -
宿主
Rabbit -
特异性
Specific for the ~29k 14-3-3 protein phosphorylated at Ser58. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
The N-terminal sequence used as antigen has high homology across a wide variety of species for all of the 14-3-3 proteins. We have not specifically tested it for reactivity against each protein in any given species, we do expect it to recognize most all of them.
-
经测试应用
适用于: WB, IHC-Frmore details -
种属反应性
与反应: Mouse, Rat, Zebrafish
预测可用于: Sheep, Chicken, Cow, Dog, Xenopus laevis, Non human primates -
免疫原
Synthetic peptide corresponding to 14-3-3 (phospho S58).
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab30554于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000. Detects a band of approximately 29 kDa.
|
|
IHC-Fr | (1) |
1/500.
|
说明 |
---|
WB
1/1000. Detects a band of approximately 29 kDa. |
IHC-Fr
1/500. |
靶标
-
功能
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. -
序列相似性
Belongs to the 14-3-3 family. -
翻译后修饰
The alpha, brain-specific form differs from the beta form in being phosphorylated (By similarity). Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.
Isoform Short contains a N-acetylmethionine at position 1. -
细胞定位
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
数据库链接
- Entrez Gene: 419190 Chicken
- Entrez Gene: 425619 Chicken
- Entrez Gene: 286863 Cow
- Entrez Gene: 287022 Cow
- Entrez Gene: 22631 Mouse
- Entrez Gene: 54401 Mouse
- Entrez Gene: 25578 Rat
- Entrez Gene: 56011 Rat
see all -
别名
- 14 3 3 antibody
- 14 3 3 protein beta antibody
- 14 3 3 protein beta/alpha antibody
see all
图片
-
All lanes : Anti-14-3-3 (phospho S58) antibody (ab30554) at 1/1000 dilution
Lane 1 : Rat brain stem lysate
Lane 2 : Rat brain stem lysate with the phosphopeptide used as the antigen
Observed band size: 29 kDa why is the actual band size different from the predicted? -
ab30554 staining 14-3-3 (phospho S58) in Mouse hippocampal tissue by Immunohistochemistry (Frozen sections). ab30554 is green, red staining is GFAP and nuclei are stained blue with DAPI.
-
All lanes : Anti-14-3-3 (phospho S58) antibody (ab30554) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate (20ug)
Lane 3 : Mouse brain homogenate (20ug)
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab30554 被引用在 2 文献中.
- Civiero L et al. PAK6 Phosphorylates 14-3-3? to Regulate Steady State Phosphorylation of LRRK2. Front Mol Neurosci 10:417 (2017). PubMed: 29311810
- Okada N et al. A novel Chk1/2-Lats2-14-3-3 signaling pathway regulates P-body formation in response to UV damage. J Cell Sci 124:57-67 (2011). WB, ICC/IF ; Human . PubMed: 21118956