Anti-14-3-3 gamma抗体[J3H10] (ab118877)

概述

  • 产品名称Anti-14-3-3 gamma抗体[J3H10]
    参阅全部 14-3-3 gamma 一抗
  • 描述
    小鼠单克隆抗体[J3H10] to 14-3-3 gamma
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-274 of Human 14-3-3 gamma (NP_036611) purified from E. coli.

  • 阳性对照
    • Human brain cortex or heart tissue. HeLa cell lysate; mouse brain lysate.

性能

应用

Our Abpromise guarantee covers the use of ab118877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Predicted molecular weight: 28 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • 组织特异性Highly expressed in brain, skeletal muscle, and heart.
  • 序列相似性Belongs to the 14-3-3 family.
  • 翻译后修饰Phosphorylated by various PKC isozymes.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 14 3 3 gamma antibody
    • 14 3 3 protein gamma antibody
    • 14 3 3 protein gamma subtype antibody
    • 14 3 3gamma antibody
    • 14-3-3 protein gamma antibody
    • 1433G_HUMAN antibody
    • 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide antibody
    • KCIP 1 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • N-terminally processed antibody
    • Protein kinase C inhibitor protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide antibody
    • Ywhag antibody
    see all

Anti-14-3-3 gamma antibody [J3H10] 图像

  • Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with ab118877at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Human Heart (formalin-fixed, paraffin-embedded) stained with ab118877 at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • All lanes : Anti-14-3-3 gamma antibody [J3H10] (ab118877) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 28 kDa

Anti-14-3-3 gamma antibody [J3H10] (ab118877)参考文献

ab118877 has not yet been referenced specifically in any publications.

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