Anti-14-3-3 gamma抗体(ab69592)

概述

  • 产品名称Anti-14-3-3 gamma抗体
    参阅全部 14-3-3 gamma 一抗
  • 描述
    兔多克隆抗体to 14-3-3 gamma
  • 特异性This antibody detects endogenous levels of total 14-3-3 gamma protein.
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from internal of human 14-3-3 gamma.

  • 阳性对照
    • Human brain tissue K562 cell extract treated with insulin Cos7 cells

性能

应用

Our Abpromise guarantee covers the use of ab69592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • 组织特异性Highly expressed in brain, skeletal muscle, and heart.
  • 序列相似性Belongs to the 14-3-3 family.
  • 翻译后修饰Phosphorylated by various PKC isozymes.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 14 3 3 gamma antibody
    • 14 3 3 protein gamma antibody
    • 14 3 3 protein gamma subtype antibody
    • 14 3 3gamma antibody
    • 14-3-3 protein gamma antibody
    • 1433G_HUMAN antibody
    • 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide antibody
    • KCIP 1 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • N-terminally processed antibody
    • Protein kinase C inhibitor protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide antibody
    • Ywhag antibody
    see all

Anti-14-3-3 gamma antibody 图像

  • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab69592 at 1/50-1/100 dilution. Right hand panel was treated with immunising peptide.
  • All lanes : Anti-14-3-3 gamma antibody (ab69592) at 1/500 dilution

    Lane 1 : K562 cell extract treated with insulin (0.01U/ml, 15mins)
    Lane 2 : K562 cell extract treated with insulin (0.01U/ml, 15mins) with peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 30 kDa
    Observed band size : 30 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of COS7 cells, using ab69592 at 1/500-1/1000 dilution. Right hand panel was treated with immunising peptide.

Anti-14-3-3 gamma antibody (ab69592)参考文献

This product has been referenced in:
  • Broadbelt KG  et al. Brainstem deficiency of the 14-3-3 regulator of serotonin synthesis: a proteomics analysis in the sudden infant death syndrome. Mol Cell Proteomics 11:M111.009530 (2012). WB, ICC/IF ; Human . Read more (PubMed: 21976671) »

See 1 Publication for this product

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Many thanks for the reply.

I am pleased to hear that the staining now seems to have improved for you and the problems initially encountered have been resolved. This information is very helpful to us in monitoring the quality of our products. Read More

Thank you for contacting us yesterday to chase up your enquiry into the Anti-14-3-3 gamma antibody (ab69592). I am sorry that your original enquiry was not dealt with as it should have been. We aim to reply to all our customers within a 24 hour period ...

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Thank you for contacting us.

I have heard back for ab63635, ab69592:

Unfortunately, for these 2 antibodies the immunogen is not available for sale.

As for ab97273 and ab43057, I am still waiting for a reply and will let y...

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Application Western blot
Sample Mouse Tissue lysate - whole (brain)
Loading amount 5 µg
Specification brain
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Dec 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"