Anti-14-3-3 epsilon抗体(ab64983)


  • 产品名称
    Anti-14-3-3 epsilon抗体
    参阅全部 14-3-3 epsilon 一抗
  • 描述
    兔多克隆抗体to 14-3-3 epsilon
  • 特异性
    This antibody detects endogenous levels of total 14-3-3 epsilon protein.
  • 经测试应用
    适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide (Human) from the C-terminal region of 14-3-3 epsilon.

  • 阳性对照
    • Extracts from HepG2 and Jurkat cells.



Our Abpromise guarantee covers the use of ab64983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ELISA 1/20000.


  • 功能
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • 序列相似性
    Belongs to the 14-3-3 family.
  • 细胞定位
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 14 3 3 E antibody
    • 14 3 3 epsilon antibody
    • 14 3 3E antibody
    • 14-3-3 protein epsilon antibody
    • 14-3-3E antibody
    • 1433E_HUMAN antibody
    • Epididymis luminal protein 2 antibody
    • FLJ45465 antibody
    • FLJ53559 antibody
    • HEL2 antibody
    • KCIP 1 antibody
    • KCIP1 antibody
    • MDCR antibody
    • MDS antibody
    • Mitochondrial import stimulation factor L subunit antibody
    • Protein kinase C inhibitor protein1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon polypeptide antibody
    • Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide antibody
    • YWHAE antibody
    see all

Anti-14-3-3 epsilon antibody 图像

  • All lanes : Anti-14-3-3 epsilon antibody (ab64983) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells
    Lane 2 : Extracts from Jurkat cells
    Lane 3 : Extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
  • ICC/IF image of ab64983 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64983, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-14-3-3 epsilon antibody (ab64983)参考文献

ab64983 has not yet been referenced specifically in any publications.

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