The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/3000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
Use at an assay dependent dilution.
功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
序列相似性Belongs to the 14-3-3 family.
细胞定位Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide antibody
Anti-14-3-3 epsilon antibody [5A5] 图像
Western blot - 14-3-3 epsilon antibody [5A5] (ab71506)
Anti-14-3-3 epsilon antibody [5A5] (ab71506) at 1/1000 dilution + Mouse brain extract at 50 µg
Secondary Goat anti-mouse secondary conjugated to HRP developed using the ECL technique
Predicted band size : 29 kDa Observed band size : 29 kDa
Anti-14-3-3 epsilon antibody [5A5] (ab71506)参考文献
This product has been referenced in:
Broadbelt KG et al. Brainstem deficiency of the 14-3-3 regulator of serotonin synthesis: a proteomics analysis in the sudden infant death syndrome. Mol Cell Proteomics : (2011).
Read more (PubMed: 21976671) »