Anti-14-3-3 beta + zeta (phospho S184 + S186)抗体(ab111856)

概述

  • 产品名称Anti-14-3-3 beta + zeta (phospho S184 + S186)抗体
    参阅全部 14-3-3 beta + zeta 一抗
  • 描述
    兔多克隆抗体to 14-3-3 beta + zeta (phospho S184 + S186)
  • 特异性ab111856 detects endogenous levels of 14-3-3 beta + zeta only when phosphorylated at serine 184 or serine 186.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from Human 14-3-3 beta + zeta around the phosphorylation site of serine 184 or serine 186 (L-N-SP-P-E).

  • 阳性对照
    • RAW264.7 cell extracts, treated with UV (15mins).

性能

应用

Our Abpromise guarantee covers the use of ab111856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 28 kDa.

靶标

  • 相关性14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exists in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.
  • 细胞定位Cytoplasmic. Melanosome (fractions from stage I to stage IV)
  • 数据库链接
  • 别名
    • 14 3 3 protein beta antibody
    • 14 3 3 protein beta/alpha antibody
    • 14 3 3 protein zeta/delta antibody
    • 14 3 3 zeta antibody
    • HS1 antibody
    • KCIP1 antibody
    • YWHAB antibody
    • YWHAZ antibody
    see all

Anti-14-3-3 beta + zeta (phospho S184 + S186) antibody 图像

  • All lanes : Anti-14-3-3 beta + zeta (phospho S184 + S186) antibody (ab111856) at 1/500 dilution

    Lane 1 : RAW264.7 cell extracts, treated with UV (15mins)
    Lane 2 : RAW264.7 cell extracts, treated with UV (15mins) with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 28 kDa

Anti-14-3-3 beta + zeta (phospho S184 + S186) antibody (ab111856)参考文献

ab111856 has not yet been referenced specifically in any publications.

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