Anti-14-3-3 beta + zeta抗体[22-IID8B] (ab12347)

概述

  • 产品名称Anti-14-3-3 beta + zeta抗体[22-IID8B]
    参阅全部 14-3-3 beta + zeta 一抗
  • 描述
    小鼠单克隆抗体[22-IID8B] to 14-3-3 beta + zeta
  • 经测试应用适用于: WB, ICC, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Rabbit, Chicken, Cow, Dog, Human, Pig, Monkey
  • 免疫原

    Lysed transformed amnion cells (AMA) (Human).

  • 阳性对照
    • HeLa cell lysate.
  • 常规说明Storage below -20°C is not recommended. For maximum product recovery centrifuge the product vial before removing cap.


    14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exist in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 50% Glycerol, PBS, 0.1mM PMSF. pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • Primary antibody说明14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exist in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.
  • 克隆单克隆
  • 克隆编号22-IID8B
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab12347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 , 30 kDa.
ICC Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.

靶标

  • 相关性14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exists in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.
  • 细胞定位Cytoplasmic. Melanosome (fractions from stage I to stage IV)
  • 数据库链接
  • 别名
    • 14 3 3 protein beta antibody
    • 14 3 3 protein beta/alpha antibody
    • 14 3 3 protein zeta/delta antibody
    • 14 3 3 zeta antibody
    • HS1 antibody
    • KCIP1 antibody
    • YWHAB antibody
    • YWHAZ antibody
    see all

Anti-14-3-3 beta + zeta antibody [22-IID8B] (ab12347)参考文献

This product has been referenced in:
  • Kaushal N  et al. The regulation of erythropoiesis by selenium in mice. Antioxid Redox Signal 14:1403-12 (2011). WB ; Mouse . Read more (PubMed: 20969477) »
  • Choi EK  et al. Calsenilin is a substrate for caspase-3 that preferentially interacts with the familial Alzheimer's disease-associated C-terminal fragment of presenilin 2. J Biol Chem 276:19197-204 (2001). Read more (PubMed: 11278424) »

See all 3 Publications for this product

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