Anti-14-3-3抗体[EPR6380] (ab125032)


  • 产品名称Anti-14-3-3抗体[EPR6380]
    参阅全部 14-3-3 一抗
  • 描述
    兔单克隆抗体[EPR6380] to 14-3-3
  • 经测试应用适用于: WBmore details
    不适用于: Flow Cyt,ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human 14-3-3 aa 200-300.

  • 阳性对照
    • HeLa, SH-SY5Y, Raji and HepG2 cell lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.




Our Abpromise guarantee covers the use of ab125032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 28 kDa).
  • 应用说明Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • 靶标

    • 功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2.
    • 序列相似性Belongs to the 14-3-3 family.
    • 翻译后修饰The alpha, brain-specific form differs from the beta form in being phosphorylated (By similarity). Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.
      Isoform Short contains a N-acetylmethionine at position 1.
    • 细胞定位Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 14 3 3 antibody
      • 14 3 3 protein beta antibody
      • 14 3 3 protein beta/alpha antibody
      • 14 3 3 protein zeta antibody
      • 14 3 3 zeta antibody
      • 14-3-3 protein beta/alpha antibody
      • 14-3-3 protein/cytosolic phospholipase A2 antibody
      • 1433B_HUMAN antibody
      • GW128 antibody
      • HS1 antibody
      • KCIP 1 antibody
      • KCIP-1 antibody
      • MGC111427 antibody
      • MGC126532 antibody
      • MGC138156 antibody
      • N-terminally processed antibody
      • Protein 1054 antibody
      • Protein kinase C inhibitor protein 1 antibody
      • Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide antibody
      • Tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide antibody
      • YWHAB antibody
      • YWHAD antibody
      • YWHAZ antibody
      see all

    Anti-14-3-3 antibody [EPR6380] 图像

    • All lanes : Anti-14-3-3 antibody [EPR6380] (ab125032) at 1/1000 dilution

      Lane 1 : Hela cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 28 kDa
      Observed band size : 27 kDa (why is the actual band size different from the predicted?)

    Anti-14-3-3 antibody [EPR6380] (ab125032)参考文献

    ab125032 has not yet been referenced specifically in any publications.

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