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RabMAb

Anti-14-3-3 alpha + beta抗体[Y62] (ab32560)

概述

  • 产品名称
    Anti-14-3-3 alpha + beta抗体[Y62]
    参阅全部 14-3-3 alpha + beta 一抗
  • 描述
    兔单克隆抗体[Y62] to 14-3-3 alpha + beta
  • 特异性
    This antibody is specific for 14-3-3 alpha + beta.
  • 经测试应用
    适用于: WB, IHC-P, ICC, IP, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in the C terminal of Human 14-3-3 alpha + beta.

  • 阳性对照
    • WB: Hela cell lysate. IHC-P: Human breast tissue slides.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab32560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa).
IHC-P Use at an assay dependent concentration.
ICC 1/500.
IP 1/40.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
  • 序列相似性
    Belongs to the 14-3-3 family.
  • 翻译后修饰
    The alpha, brain-specific form differs from the beta form in being phosphorylated.
    Isoform Short contains a N-acetylmethionine at position 1.
  • 细胞定位
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 14 3 3 alpha antibody
    • 14 3 3 protein beta/alpha antibody
    • 14-3-3 protein beta/alpha antibody
    • 1433B_HUMAN antibody
    • Brain protein 14 3 3 beta isoform antibody
    • GW128 antibody
    • HS 1 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • N-terminally processed antibody
    • Protein 1054 antibody
    • Protein kinase C inhibitor protein 1 antibody
    • YWHAA antibody
    • YWHAB antibody
    see all

图片

  • Anti-14-3-3 alpha + beta antibody [Y62] (ab32560) at 1/1000 dilution + Hela cell lysate.

    Predicted band size : 30 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
  • Ab32560, at a 1/500 dilution, staining 14-3-3 alpha + beta in paraffin embedded human breast tissue by Immunohistochemistry.

文献

This product has been referenced in:
  • Gao K  et al. Front-signal-dependent accumulation of the RHOA inhibitor FAM65B at leading edges polarizes neutrophils. J Cell Sci 128:992-1000 (2015). WB, IP ; Human . Read more (PubMed: 25588844) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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