10x Tris缓冲液pH 10.0 (ab64222)

概述

  • 产品名称10x Tris缓冲液pH 10.0
    参阅全部 Tris buffer 试剂
  • 经测试应用适用于: IHC-Pmore details
  • 常规说明Recommended Procedure: Microwave
    1. Deparaffinize and rehydrate tissue sections.
    2. Dilute the product 1:10 with deionized water.
    3. Immerse sections in coplin jar filled with target retrieval solution, microwave on high power until boiling.
    4. Keep the slides warm by heating for 10min at low power.
    5. Let coplin jar sit in microwave for at least 20min.
    6. Remove and rinse with buffer.
    7. Proceed with staining.

性能

  • 相关性Tris buffer is used on formalin-fixed, paraffin-embedded tissue sections mounted on glass slides for target retrieval prior to immunohistochemistry (IHC) procedures. Target retrieval prior to IHC procedures obtains positive results, or results in an increase in staining intensity with many primary antibodies.
  • 别名
    • Trishydroxymethylaminomethane

应用

Our Abpromise guarantee covers the use of ab64222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent dilution.
Use at 1x Dilution.

10x Tris Buffer pH 10.0 (ab64222)参考文献

ab64222 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"