10x EDTA缓冲液pH 8.0 (ab64216)

概述

  • 产品名称10x EDTA缓冲液pH 8.0
    参阅全部 EDTA buffer 试剂
  • 经测试应用适用于: IHC-Pmore details
  • 常规说明Recommended Procedure: Microwave
    1. Deparaffinize and rehydrate tissue sections.
    2. Dilute the product 1:10 with deionized water.
    3. Immerse sections in coplin jar filled with target retrieval solution microwave on HIGH power until boiling.
    4. Keep the slides warm by heating for 10min at LOW power.
    5. Let coplin jar sit in microwave for at least 20min.
    6. Remove and rinse with buffer.
    7. Proceed with staining.

性能

  • 相关性EDTA buffer is used on formalin-fixed, paraffin-embedded tissue sections mounted on glass slides for target retrieval prior to immunohistochemistry (IHC) procedures. Target retrieval prior to IHC procedures obtains positive results, or results in an increase in staining intensity with many primary antibodies.
  • 别名
    • Ethylenediaminetetraacetic acid buffer

应用

Our Abpromise guarantee covers the use of ab64216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent dilution.

实验方案

10x EDTA Buffer pH 8.0 (ab64216)参考文献

ab64216 has not yet been referenced specifically in any publications.

Product Wall

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I have checked with my colleagues and the concentration is 10 mM EDTA. We will update the datasheet accordingly very soon.

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ab64216 is supplied with a 10 mM solution of EDTA which is a 10X stock solution. The working concentration is 1mM EDTA. I apologize for any confusion. Please do not hesitate to contact me with additional questions or concerns.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"