10X Blocking缓冲液(ab126587)
概述
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产品名称
10X Blocking缓冲液 -
经测试应用
适用于: WB, ELISA, In-Cell ELISA, ICC/IFmore details
不适用于: Flow Cyt -
常规说明
Protein blocking buffer (pH 7.5) (ab126587) for serum-free blocking of non-specific antibody binding in ICC, ELISA and western blot. BSA-free
Preparation: Dilute to 1X in deionized water or PBS (e.g. 1mL 10X blocking buffer + 9mL water/PBS). Diluting in PBS instead of water makes a somewhat more stringent blocking buffer due to the presence of salt in the PBS. Blocking Buffer is pH 7.5. The user should determine empirically whether dilution in water or PBS is most appropriate for the assay at hand. Use diluted block solution promptly.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at Room Temperature. The product can be stored for up to 12 months. -
存储溶液
Constituent: 100% Casein Blocking Buffer 10x -
Concentration information loading...
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126587于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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In-Cell ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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说明 |
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WB
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
In-Cell ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
图片
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Primary :
All Lanes : top membranes with Anti AMPK pThr172 + Thr183 (ab133448) at 1/1000. Bottom membranes with Anti AMPKa (ab110036) at 1/1000
Lane 1 = AMPKa1 protein
Lane 2 = C2C12 cells treated with AICAR
Lane 3 = untreated C2C12 cells
Secondary: Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) and Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1:10,000 developed using the ECL technique.
Performed under reducing conditions (50mM DTT – Sample heated at 60ºC). Predicted band size for AMPKa: 64 kDa Observed band size for AMPKa: 64 kDa
Left membrane All blocking steps = 1X blocking reagent ab126587 diluted in PBST
Right membrane All blocking steps = 5% Milk in PBST Exposure time : 5 minutes
Primary :
All Lanes : top membranes with Anti AMPK pThr172 + Thr183 (ab133448) at 1/1000. Bottom membranes with Anti AMPKa (ab110036) at 1/1000
Lane 1 = AMPKa1 protein
Lane 2 = C2C12 cells treated with AICAR
Lane 3 = untreated C2C12 cells
Secondary: Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) and Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1:10,000 developed using the ECL technique.
Performed under reducing conditions (50mM DTT – Sample heated at 60ºC). Predicted band size for AMPKa: 64 kDa Observed band size for AMPKa: 64 kDa
Left membrane All blocking steps = 1X blocking reagent ab126587 diluted in PBST
Right membrane All blocking steps = 5% Milk in PBST Exposure time : 5 minutes
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab126587 被引用在 18 文献中.
- Yang JM et al. Gradient Waveguide Thickness Guided-Mode Resonance Biosensor. Sensors (Basel) 21:N/A (2021). PubMed: 33430392
- Dang TM et al. Optimization of the isolation procedure and culturing conditions for hepatic stellate cells obtained from mouse. Biosci Rep 41:N/A (2021). PubMed: 33350435
- Yokoe I et al. Lipid bubbles combined with low-intensity ultrasound enhance the intratumoral accumulation and antitumor effect of pegylated liposomal doxorubicin in vivo. Drug Deliv 28:530-541 (2021). PubMed: 33685314
- Ho CNQ et al. Simulated Microgravity Inhibits the Proliferation of Chang Liver Cells by Attenuation of the Major Cell Cycle Regulators and Cytoskeletal Proteins. Int J Mol Sci 22:N/A (2021). PubMed: 33925309
- Chou LY et al. Discoidin Domain Receptor 1 Regulates Runx2 during Osteogenesis of Osteoblasts and Promotes Bone Ossification via Phosphorylation of p38. Int J Mol Sci 21:N/A (2020). PubMed: 33003599