10kD Spin Column (ab93349)
概述
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产品名称
10kD Spin Column -
产品概述
The 10kD Spin Columns (ab93349) are disposable ultrafiltration devices for the concentration or deproteinization of biological samples.
They can be used in either a swing bucket or fixed angle rotors which accept 2.0 mL centrifuge tube at maximum speed 10000 x g (5-10 mins). The vertical membrane design and thin channel filtration chamber minimizes membrane fouling and provides high speed concentration, even laden solutions. It is specifically designed with low internal surface and membrane area in order to achieve superior recovery from dilute solution.
Maximum initial sample volume is 500µL. The dead stop volume is approximately 20-25µL.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called 1997 10K Spin Column. 1997-25 is the same size as the 25 unit size of ab93349.
If working with cell or tissue lysates, we generally recommend the TCA Sample Deproteinization Kit or using a PCA/KOH Deproteinization protocol. If it is critical to use a spin column with a cell or tissue lysate then, to avoid blocking the filter, first centrifuge the lysate samples at ~14,000 rpm for 10-20 mins to pellet debris, and then use the resulting supernatant with the spin column.
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经测试应用
适用于: Purificationmore details
性能
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存放说明
Store at room temperature. Please refer to protocols. -
组件 25 units 25 units Concentrator cup: Polycarbonate 25 units 25 units Filtrate vessel: Polypropylene 25 units 25 units Membrane: 10,000 MWCO Polyethersulfone 25 units 25 units -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab93349于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Purification |
Use at an assay dependent concentration.
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说明 |
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Purification
Use at an assay dependent concentration. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (27)
ab93349 被引用在 27 文献中.
- Gong J et al. The pentose phosphate pathway mediates hyperoxia-induced lung vascular dysgenesis and alveolar simplification in neonates. JCI Insight 6:N/A (2021). PubMed: 33497360
- Nayak G et al. Adaptive Thermogenesis in Mice Is Enhanced by Opsin 3-Dependent Adipocyte Light Sensing. Cell Rep 30:672-686.e8 (2020). PubMed: 31968245
- Ortiz-Masiá D et al. Succinate Activates EMT in Intestinal Epithelial Cells through SUCNR1: A Novel Protagonist in Fistula Development. Cells 9:N/A (2020). PubMed: 32365557
- Feng D et al. Nicotinamide Phosphoribosyltransferase (Nampt)/Nicotinamide Adenine Dinucleotide (NAD) Axis Suppresses Atrial Fibrillation by Modulating the Calcium Handling Pathway. Int J Mol Sci 21:N/A (2020). PubMed: 32629939
- Sharma I et al. Myo-inositol Oxygenase (MIOX) Overexpression Drives the Progression of Renal Tubulointerstitial Injury in Diabetes. Diabetes 69:1248-1263 (2020). PubMed: 32169892