Anti- Inosine triphosphate pyrophosphatase抗体(ab79967)

概述

  • 产品名称Anti- Inosine triphosphate pyrophosphatase抗体
    参阅全部 Inosine triphosphate pyrophosphatase 一抗
  • 描述
    兔多克隆抗体to Inosine triphosphate pyrophosphatase
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Inosine triphosphate pyrophosphatase.

  • 阳性对照
    • This antibody gave a positive signal in HepG2 Whole Cell Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab79967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
WB
  • 应用说明ICC/IF: Use at a concentration of 5 µg/ml.
    WB: Use at an assay dependent dilution. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Hydrolyzes ITP and dITP to their respective monophosphate derivatives. Xanthosine 5'-triphosphate (XTP) is also a potential substrate. May be the major enzyme responsible for regulating ITP concentration in cells.
    • 组织特异性Ubiquitous. Highly expressed in heart, liver, sex glands, thyroid and adrenal gland.
    • 疾病相关Defects in ITPA are the cause of inosine triphosphate pyrophosphohydrolase deficiency (ITPA deficiency) [MIM:147520]. It is a common inherited trait characterized by the abnormal accumulation of inosine triphosphate (ITP) in erythrocytes and also leukocytes and fibroblasts. The pathological consequences of ITPA deficiency, if any, are unknown. However, it might have pharmacogenomic implications and be related to increased drug toxicity of purine analog drugs. Three different human populations have been reported with respect to their ITPase activity: high, mean (25% of high) and low activity. The variant Thr-32 is associated with complete loss of enzyme activity, may be by altering the local secondary structure of the protein. Heterozygotes for this polymorphism have 22.5% of the control activity: this is consistent with a dimeric structure of the enzyme.
    • 序列相似性Belongs to the HAM1 NTPase family.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C20orf37 antibody
      • dJ794I6.3 antibody
      • HLC14-06-P antibody
      • Inosine triphosphatase (nucleoside triphosphate pyrophosphatase) antibody
      • Inosine triphosphatase antibody
      • inosine triphosphatase-A antibody
      • Inosine triphosphate pyrophosphatase antibody
      • Inosine triphosphate pyrophosphohydrolase antibody
      • Itpa antibody
      • ITPA_HUMAN antibody
      • ITPase antibody
      • My049 antibody
      • My049 protein antibody
      • Non canonical purine NTP pyrophosphatase antibody
      • Non standard purine NTP pyrophosphatase antibody
      • NTPase antibody
      • nucleoside triphosphate diphosphatase antibody
      • Nucleoside triphosphate pyrophosphatase antibody
      • OK/SW-cl.9 antibody
      • OTTHUMP00000030094 antibody
      • OTTHUMP00000160459 antibody
      • Putative oncogene protein hlc14-06-p antibody
      see all

    Anti- Inosine triphosphate pyrophosphatase antibody 图像

    • Anti- Inosine triphosphate pyrophosphatase antibody (ab79967) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 21 kDa
      Observed band size : 24 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 125 kDa,70 kDa,75 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 10 minutes
    • ICC/IF image of ab79967 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79967, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml.

    Anti- Inosine triphosphate pyrophosphatase antibody (ab79967)参考文献

    ab79967 has not yet been referenced specifically in any publications.

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