Anti-ß-phosphoglucomutase抗体(Biotin) (ab34541)

概述

  • 产品名称Anti-ß-phosphoglucomutase抗体(Biotin)
    参阅全部 ß-phosphoglucomutase 一抗
  • 描述
    山羊多克隆抗体to ß-phosphoglucomutase (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: Immunomicroscopy, WB, ELISA, Dot blotmore details
  • 种属反应性
    Reacts with Lactococcus lacti
  • 免疫原

    Full length protein [Lactococcus lacti]

  • 常规说明

    Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule

性能

应用

Our Abpromise guarantee covers the use of ab34541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Immunomicroscopy
WB
ELISA
Dot blot
  • 应用说明Dot: Use at an assay dependent dilution.
    ELISA: 1/4000 - 1/20000. This product has been assayed against 1.0 ug of beta Phosphoglucomutase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature.
    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 62 kDa.
    Suitable for antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性ß-phosphoglucomutase is an enzyme that transfers a phosphoryl group on a glucose monomer from the 1' to the 6' position in the forward direction or the 6' to the 1' position in the reverse. Specifically, it converts ß-D-glucose-1-phosphate to ß-D-glucose-6-phosphate. This enzyme participates in both the breakdown and synthesis of glucose. Maltose metabolism in Lactococcus lactis involves the conversion of beta-glucose 1-phosphate to glucose 6-phosphate, a reaction which is reversibly catalysed by a maltose-inducible and glucose-repressible beta-phosphoglucomutase (beta-PGM). Alpha-PGM is expressed constitutively. ß-phosphoglucomutase is a member of the haloacid dehalogenase superfamily of hydrolase enzymes. The enzyme from Lactococcus lactis has been extensively characterised including a remarkable crystal structure which traps the pentacoordinate transition state.
    • 细胞定位Cytoplasmic
    • 别名
      • Beta PGM antibody
      • Beta-phosphoglucomutase antibody
      • Glucose phosphomutase 1 antibody
      • Glucose phosphomutase antibody
      • PGM antibody
      see all

    Anti-ß-phosphoglucomutase antibody (Biotin) (ab34541)参考文献

    ab34541 has not yet been referenced specifically in any publications.

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